Enzymatic saccharification of biomass was done by incubating 1 g of pretreated rice straw with different loading of crude unprocessed cellulase and BGL (produced in “Microorganisms, inoculum preparation, and hydrolytic enzyme production” section) preparations with Tween 80 surfactant at 45°C, in stoppered 250-ml flasks, total volume of 50 ml made up with 50 mM citrate buffer (pH 4.8).