with high activities in both static and stirred
cultures (Fig. 5). The LiP , MnP and Laccase by agitation with maximum values of 832 U/L
(Fig. 5a), 250 U/L (Fig. 5b) and 83.4 U/L. Agitation increased the
oxygen and nutrient transfer rate which was decisive for biomass
growth and enzymes production and activities. Therefore, the high
ITE decolourization was corroborated with the extracellular peroxidases
and mycelium growth, which favor the biodegradation of complex molecules
The time courses of LiP and MnP production by C. globosum in both static and stirred conditions showed highest activities in 3
days and 5 days of incubation, respectively and then decreased. However, the Laccase enzyme was measured in the supernatant until the 8th hour of culture with a maximum activity in 3 daysPasti-Grigsby et al. (1992) suggested that the maximum time taken
for decolourization varies with the nature of dyes molecular. Besides,
the longest time taken for decolourization is the result of the
production of extracellular peroxidases, which are available only
after 2e3 days of the growth of the fungus.
Kanmani et al. (2011) and Da^assi et al. (2012) showed that
Laccase was involved in decolourization and biodegradation of
textile effluent containing dyes. However, Asgher et al. (2010)
observed that MnP and LiP were the key enzymes responsible for
the decolourization process. They have also suggested that decolourization
starts on the first day and reaches its maximum rate
during the third and fourth day and declined. Therefore, C. globosum
IMA1 was found to be suitable for the decolourization and
biodegradation of ITE producing ligninolytic enzymes such as LiP,
MnP and Laccases.