of 50 mM Tris HCl buffer, pH 7.4 containing 0.1 M NaCl and 0.1% Triton X-100 and 1 vol. of fine glass powder by using a mortar and pestle. The crude extract then was sonicated for 20 s. The
homogenate was centrifuged at 3000g for 5 min, and the resulting supernatant was stored at – 20 C until further use.