Two hybrid cultivars of sunflower,high oleic acid (Pacific 22) and low oleic acid content in seeds (Pacific 77), were cultivated in the experimental field at the Suranaree University of Technology,Nakhon Ratchasima, Thailand. At maturity stage (120 days after planted), 20 plants from each cultivar were randomly sampled.Seed samples were ground into powder using a grinder and passed through an 80-mesh sieve. To eliminate moisture interference, the samples were dried at 60 °C for 24 h before chemical analysis and FTIR sampling. After grinding, the samples were divided into two subsamples.One subsample was used for GC analysis (reference analysis) and another sub-sample was used to collect FTIR spectra.