Agar well diffusion was described as an assay with a relatively low sensitivity , because the samples were further diluted as soon as they diffused into the agar. However, this procedure is one of the most employed to determine antibacterial activity of honey, because it allows the study of a vast number of sample.Our results have shown that in order to get proper and accuratedata about antibacterial activity of honeys against Staphylococcusaureus, agar well diffusion should be combined with other proce-dure such as broth dilution, in order to know MIC. Other possibilityis comparing agar well diffusion with phenol standard solutions,so that antimicrobial activity is expressed as concentration of phe-nol with equivalent activity