Control
culture medium, called α-MEM+, consisted of α-MEM (pH
7.2–7.4) supplemented with 3.0 mg/ml bovine serum
albumin (BSA), ITS (10 μg/ml insulin, 5.5 μg/ml transferrin,
5 ng/ml selenium), 2 mM glutamine, 2 mM hypoxanthine
and 50 μg/ml ascorbic acid under mineral oil.