Cellulose hydrogel films from bamboo fibers dissolved by three different methods were obtained. Depending of the dissolving method used, hydrogel film exhibited lower tensile strength, elongation and contact angle in the case of NaOH and NaOH/urea systems. On the other hand, very good cytocompatibility was observed in the hydrogel films elaborated with DMAc/LiCl solution. It was proved that the dissolving method used for the preparation of the cellulose solution affects the properties of the obtained films. These results suggest that hydrogel films elaborated with cellulose solution prepared with DMAc/LiCl showed better possibility of usage for tissue engineering scaffold.