As an initial material to work from the meristem explants, apical buds and meristem with size up to 3 mm for the buds, and 0.5 mm for the meristem were used. From the non meristematic explants were used whole cotyledones or parts from cotyledons, hypocotyls, nods, internods and peper anthers for haploid regenerants. All initial explants isolated from seeds were previously subject to sterilization.