All the quantification of FA was performed spectrophotometrically following the method of
Marshall, Orten, and Smith. However, some technical improvisations were made in the
original protocol that did not cause any significant difference in the measured values of FA.
The technical comparison of the procedure mentioned in the original work and followed in the
present investigation is shown in Table 1. The outcome of the technical improvisation of the
existing technique could be evaluated in terms of cost (volumetric reduction of reagents used)
and time effectiveness (total estimation time). However, the chemical requirement was the
same in both cases. As can be summarized from Table 1, there was an almost fourfold decrease
in the total time of estimation in the improvised technique. Practically, there was no drastic
change in the FA concentration determined following both procedures