Conclusion This is the first report which evaluates the effects of solvent and extraction method on various phenolic extracts of O. ficus-indica flowers. The method of extraction, as well as the extracting sol-vent, significantly affected extraction yield, total polyphenol, total falvonoid contents and antioxidant activities.The screening by various extraction solvents showed that the most efficient solvents for polyphenol extraction were the polar solvents. Based on the results of total phenolic, total flavonoid contents and extract yield, it can be concluded that the best extract-ing solvent was MeOH using the soxhlet method.The antioxidant potential of O. ficus-indica flowers extracts determined by the different tests (DPPH radical scavenging, reducing power and -carotene bleaching assays) showed the same trends: the antioxidant potential is more important when using polar solvents and Soxhlet extraction than those extracted by mac-eration. The methanolic and aqueous extracts show significant antioxidant activities, suggesting that they are the best effective solvent for extraction of antioxidants from O. ficus-indica flowers.These results indicate that proper extraction from O. ficus-indica flowers, by suitable solvents and methods, is important to obtain natural antioxidant that may have beneficial health effects. In this regard, these flowers can be used in the field of pharmaceutical and as preservatives ingredients in the food.Further investigations are needed to elucidate the chemical identification of the bioactive compounds of the studied extracts.Moreover, studies using in vivo models will help to confirm the beneficial quality of these extracts.