The recombinant
clone designated as βLR16 (Table 1) was recovered only on carbenicillin (50μg ml-1) after
growth at 24°C. Subsequent growth of the parent clone and subclones was in Luria-Bertani
(LB) medium at 24–28°C. Growth media was amended with tetracycline at 20 μg ml-1 or kanamycin
at 20 μg ml-1 for plasmid maintenance where appropriate. The DNA sequence of the
βLR16 DNA insert was previously submitted to GenBank (EU408353.1).