For root induction, long (4–5 cm) and healthy shoots were selected from the multiplication stage of the cultures. The unwanted parts were removed from the shoots and placed on the semi-solid full strength, half- strength and 1/4th strength MS medium augmented with indole-3 butyric acid (IBA) and indole-3 acetic acid (IAA) concentrations (ranging from 0.5 to 5.0 mg/L). The
cultures were maintained under normal light conditions for 2 days for pre root induction and then maintained at 25 ± 2 ◦C temperature in diffused light (15–20 mol m−2 s−1 SFP) field for 5 weeks.After 5 weeks, the in vitro rooted plantlets were carefully taken from the culture tubes and washed with sterilized distilled water.The response (%) of rootinduction, number and length (cm) of roots and callus significance were measured.