Recombinant silk protein and chimeras were purified
by Ni-NTA affinity chromatography. The recombinant silk protein
and chimeras eluted from the Ni-NTA resin were dialyzed against
100 mM phosphate buffer (pH 5.5) for 1 day followed by distilled
water for 3 days using cellulose ester snake skin tubing with
MWCO 10,000 Da (Thermo Scientific, MA).