UV–visible spectra of solubilized TherJR_0333 in 10 mM potassium
phosphate buffer (pH 7.6) with 100 mM KCl and 0.05% DDM
were acquire on a Shimadzu UV-1800 spectrophotometer in the
range of 250–800 nm at room temperature. Reduced spectra were
obtained by adding an excess of sodium dithionite (Sigma) to the
oxidized sample