The activity of carbonic anhydrase growth of strain ISTS2 isolated from bacterial community enriched in sodium bicarbonate (100 mM) in the chemostat and carbon dioxide further grew in minimal salt medium with sodium bicarbonate and carbon dioxide (5%) as sole carbon source indicated that microbial growth and enzyme production was gradually increased from 6 to 48 h and then started to decline (Fig. 1). Higher activity of carbonic anhydrase in experiment revealed that the bacterial strain isolated was efficient for CO2 sequestration because the presence of carbonic anhydrase helps to development of carbon concentrating mechanism inside the bacteria. It dehydrates cytosolic pool of bicarbonate inside the carboxysome and present it to (RuBisCO) during the sequestration process. In these way bacteria gets acquainted to sequester more CO2 (Ramanan et al., 2009). Hence, activity of carbonic anhydrase is an important factor for CO2 sequestration.