Kinetics of extractions were studied at four different temperatures
(Te), namely 60 C, 100 C, 130 C and 160 C for extraction
times (te) ranging from 5 min to 120 min at a material to solvent
ratio (M/S) of 1/20 g/mL. The extraction kinetics at 130 C were also
observed at the M/S ratio of 1/10 g/mL and 1/30 g/mL. The extraction
suspension was filtered and the obtained liquid extract along
with 50 mL of hexane was introduced into a separation funnel
which was then shaken rigorously for 3 min. The formed emulsion
was centrifuged (Eppendorf, Germany) at 11,000 rpm for 2 min in
order to separate the two phases, both of which were then collected
and evaporated until dryness. Both oil (OE) and water soluble
extract (WSE) samples were stored at 20 C until further use.