The use of anesthetics in Angelfish (Pterophyllum scalare) has not been fully explored. The aim of this study is to
determine the lowest effective dose (LED) of three anesthetics (2-phenoxyethanol, clove oil and tricaine
methanesulphonate (MS-222)) and their effect after multiple exposures at 24 and 48 h. Each agent was tested
on ten juvenile angelfish at four different doses. Considering the effect criteria of complete anesthetic induction
time within 3 min and recovery time within 5 min, LEDs were established at 800 mg L−1 for 2-phenoxyethanol,
100 mg L−1 for clove oil and 140 mg L−1 for MS-222. Different anesthesia and recovery times should be considered
when repeated exposures during consecutive days are performed on juvenile angelfish