Pyrosequencing analysis was performed after three months of
MFC operation. The available sample volume of Barnett produced
water was limited (∼0.5 L) and allowed only for the acclimation and
first feed of the MFC. This was enough to allow the bacteria from
produced wastewater to colonize the anode and generate electricity.
In the subsequent cycles synthetic hypersaline media was
used as replacement; which contained sodium benzoate (1 g/L),
MgCl2. 6H2O (30 g/L), KCl (7 g/L), Yeast (8.5 g/L), peptone (1.7 g/L),
casamino acids (1.7 g/L) and 100 g/L NaCl. The pH was adjusted to
7.5. The original produced water from Barnett Shale, the bacteria
colonizing the anode and bacteria from the bulk fluid of the MFC
were analyzed by pyrosequencing following the method previously
described [10].