. Total polyphenol was analysed by spectrophotometer at 700 nm
using Folin–Ciocalteau reagent by the method described by
Singleton et al. (1999) (10%, v/v) with some modifications and
expressed as gallic acid equivalent using the equation based on the
calibration curve: y = 0.147x, R
2
= 0.974, where x is the gallic acid
equivalent (mg/100 g) and y is the absorbance