Air was used for sparging the bioreactor at a constant flow of 0.25 vvm (vol. gas (vol. liquid-1 min-1), dissolved oxygen tension (D.O.) was maintained minimal at 25 % coupled with stirred speed of 400– 1000 rpm, pH was initiated at 3.5 adjusted by 1.5 M H3PO4 (acid) and kept at 2.3 by addition of 4 M KOH (base).
Before inoculation of the fermentors, A. terreus strain was pre-cultured overnight in 500 ml baffled Erlenmeyer flasks with 100 ml of glucose production medium (107 spores/ml), 150 rpm, 37 C.