3.2. Processing of candidate RMs and material properties
Three hundred kg of cheese (starting amount) was processed to
three candidate RM batches as described in Section 2.3. The dry
mass in the cheese was 60.3%, indicated by the mass loss during
freeze-drying (39.7%). Thus, 15.1 g of cheese powder and 9.9 g of
water needed to be mixed in order to reconstitute the sample
(25 g ‘‘cheese’’ as stipulated as starting sample size for testing
(Commission Regulation 1441/2007)). Three samples randomly
picked from the bottled material were subjected to particle size
analysis (PSA) over a range of 0.5–1000 lm using laser diffraction.
The result for material A was an average particle size of 96 lm
(90% of particles smaller than 321 lm), for B an average particle
size of 93 lm (90% of particles smaller than 305 lm) and of C an
average particle size of 105 lm (90% of particles smaller than
340 lm). Values are averages of six determinations. In addition,
the residual water content in the final materials was determined
by Karl Fischer titration, yielding 2.1 ± 0.1 g/100 g (n = 9) for A,
2.3 ± 0.1 g/100 g (n = 9) for B, and 2.3 ± 0.1 g/100 g (n = 9) for C.