Conclusions
To sum up, GISH and RFLP analyses are useful for identifying intergeneric hybrids derived from Ascocenda John De Biase ‘Blue’ (female parent) and Phalaenopsis Chih Shang’s Stripes (male parent) and evaluating the genetic inheritance of these hybrids. The results showed that the GISH and RFLP method can effectively identify all F1 hybrid seedlings are intergeneric hybrids. Moreover, the use of PCR-RFLP analysis to assess the inheritance of the putative hybrids should be carefully evaluated.