Material and Methods
Preparation of modified diesel oil medium: The modified
diesel oil medium comprised of 0.7 gm K2HPO4, 0.1 gm
(NH4)2SO4, 0.3 gm KH2PO4, 0.3 gm MgSO4 7H2O, 2.2 gm agar
– agar5. The mineral components of the medium were dissolved
in 100 ml of distilled water and mixed with 2 ml of Gulf diesel
engine oil. The medium was autoclaved at 121oC for 15 min.