The development of an Alamar Blue assay to evaluate plant cell viability and/or proliferation in
tomato cell suspension was studied [70]. Comparisons were made with the more commonly used
2,3,5-triphenyltetrazolium chloride (TTC) assay. Compared to the TTC viability assay, the Alamar
Blue assay was: (i) more rapid since no organic extraction of the product was required, (ii) non-toxic to the cells which facilitated downstream analysis of the same cell culture, (iii) different quantification methods can be used, and (iv) more sensitive at low percentages of cell viability. However, the disadvantages of the AB assay included a lower substrate capacity than the TTC assay