was carried out and compared with the three times repeated extraction. TS was heated to separate TSH easily, then the partly dried TSH was obtained and used for the extraction. The content of polymeric PA in crude TSE was analyzed by HPLC and determined using acTSE as a standard. The results are summarized in Table 2. The content of polymeric PA in
fresh TSH reached about 39% (w/w), whereas in crude TSE prepared by one pot extraction with ethanol/water
(3:2, v/v) at 60оC for 24 hours it was very high (about 94%, w/w). The recovery of polymeric PA from TSH with