2. Materials and methods
2.1. Microbial cultures
The recall-associatedM. circinelloides strain isolated fromspoiled yogurt
and two clinical isolates were generously provided by the Joseph
Heitman at Duke University (Durham, NC). M. circinelloides strains
NRRL 3615, 3614, and 3627were obtained fromUSDA's Agricultural Research
Service collection (Table 1). All isolates were maintained as frozen
stock cultures and plated on Potato Dextrose Agar (Becton,