The best medium for callus induction shown in the medium supplemented with 0.4 mg/l Kinetin and 4.0 mg/l 2, 4-D. The gradually acclimatized plants were successfully transferred to the out-air conditions and a high survival rate (95%) was recorded finally the successfully protocol for in vitro culture of Ficus carica has also supported the research on transgenic plants obtained by genetic transformation.