Cytocompatibility analysis on the scaffold
After seeding on the 3-D scaffolds with corneal cells, SEM
demonstrated the orientation and morphology of corneal
cells and their processes in parallel with the longitudinal
guidance channels (Fig. 2). After seeding on the scaffolds,
corneal cells rapidly attached to the scaffold surface
(Fig. 2A). The cells extended long processes and migrated
into the acellular decalcified scaffold (Fig. 2B). After 7
days of culture, corneal cells were shown to migrate deep
into the scaffold and demonstrate a homogeneous and
dense growth pattern with an orientation along the
channels, indicating that the cells grow and migrate along
the guidance channels structure of the scaffolds