The AFM1 standard was supplied by R-Biopharm Rhone (Glasgow,
Scotland) (Afla standard solution, Cat No. P42). The bottle
consists of 1 mg AFM1 in one ml of methanol. Stock solution standard
was diluted with freshly in HPLC mobile phase consisting of wateracetonitrile-
methanol (6.8/2.4/0.8, v/v/v) to obtain concentration of
5 ng ml1. From this intermediate solution, a series of working
standards from 0.05, 0.25, 0.5, 1 and 2 ng ml1 were prepared.