2.5. Addition of lycopene and BHA in anhydrous cow milk fat
Lycopene extract was added to anhydrous cow milk fat at levels of 30, 60, 90, 120 and 150 ppm. The concentration of lycopene extract to be added in anhydrous cow milk fat was decided on the basis of antioxidant activity and its effect on sensory scores for color. Small quantity of anhydrous cow milk fat was melted (60–70 °C) and the weighed amount of crude lycopene extract was added in anhydrous cow milk fat and mixed properly. BHA was added in hot anhydrous cow milk fat (60–70 °C) at a level of 0.02% (200 ppm) and mixed properly.