Medium Optimization
For acetic acid medium optimization experiments, the strain was cultivated on medium No.
3 which composed of (g/L): glucose, 30; polypeptone, 2; yeast extract, 5 with different
glucose concentrations up to 120 g/L and incubated on rotary shaker under the same
conditions above mentioned. Subsequently, cultivations were conducted at different yeast
extract concentrations (0-15 g/L), followed by further investigation on the effect of peptone
concentration (0-6 g/L) on cell growth and acetic acid production.
Medium OptimizationFor acetic acid medium optimization experiments, the strain was cultivated on medium No.3 which composed of (g/L): glucose, 30; polypeptone, 2; yeast extract, 5 with differentglucose concentrations up to 120 g/L and incubated on rotary shaker under the sameconditions above mentioned. Subsequently, cultivations were conducted at different yeastextract concentrations (0-15 g/L), followed by further investigation on the effect of peptoneconcentration (0-6 g/L) on cell growth and acetic acid production.
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