For preparation of ultrasonicated samples (Sample 7x–11x), a 13 mm ultrasonic probe coupled to ultrasonic processor with a res-onance capacity of 20 ± 50 kHz (Cole Palmer, USA) was used. 100 g of fresh water hyacinth sample was loaded in glass beaker (diam-eter 10 cm, height 16 cm) containing 1000 ml distilled water. The ultrasonicator probe was submerged in the beaker at maximum (but not touching the bottom of the beaker) to ensure uniform irra-diations on the sample and have direct contact with the mixture inside the beaker. The ultrasonication pretreatment processes were carried out at different ultrasonicator power of 20%, 40%, 60%, 80% and 100%, each for 30 min. During the 30 min of irradiation