Images were analyzed using PDQuest v7.3.1 (Bio-Rad; Hercules, CA). After automated spot detection and matching, replicate groups were manually assigned. The group consensus tool was then used to manually examine each spot considered in the analysis. Only spots that could be reliably detected on all gels were considered further. A student’s t-test was then performed to evaluate which spots showed a difference in accumulation (p < 0.001). The integrated excision tool was then used to control a Proteome Works Spot Cutter (Bio-Rad, Hercules, CA) and excise spots of interest