The operating conditions for a shake-flask culture of Ganoderma lucidum were studied. Of the carbon sources examined, brown sugar and
malt extract most effectively promoted mycelial growth and the production of polysaccharide, while yeast extract and skim milk were the
most effective nitrogen sources. Adding safflower seed oil to the medium significantly promoted the formation of mycelium. Additionally,
supplying CaCO3 promoted both the mycelial growth and the polysaccharide production.