5. Determination of Levels of Total Tannins Manufacture of Standard Solution. Carefully weighed 10 mg gallic acid, then dissolved in distilled water and paid back the volume up to 10 ml to obtain a level of 1 mg / ml as a stock solution. And the stock solution pipetted their respective ¬ 10, 15, 20, 25 and 30 ml, was added to 0.2 ml of Folin Ciocalteu (after diluted with distilled water 1:1), homogeneously mixed for 10 seconds and then allowed to stand for 5 minutes.