PCR was conducted with AccPrime Polymerase High Fidelity (Invitrogen,USA)in a 40 reaction with the following conditions: initial denaturation for 3 min at 98 followed by 32 cycles of 1 min denaturation at 94 and 2 min annealing/elongation at 60.
PCR was conducted with AccPrime Polymerase High Fidelity (Invitrogen,USA)in a 40 reaction with the following conditions: initial denaturation for 3 min at 98 followed by 32 cycles of 1 min denaturation at 94 and 2 min annealing/elongation at 60.