2.2. Rooting of in Vitro Shoots
Two cm long shoots were excised and transferred into test tubes containing 10 mL 1/2 strength MS medium
supplemented with varying levels of indole acetic acid (IAA), indolebutryic acid (IBA), and naphthaleneacetic acid (NAA) to induce rooting (Table 1). Cultures were incubated in dark for one week and then transferred to
light at 25˚C ± 2˚C with a 16 h photoperiod (50 μmol∙m−2∙s−1). Test tubes were arranged in a completely randomized
design with ten replicates per treatment and the experiment was repeated three times. Destructive root
count was performed after six weeks and the number of roots produced by each shoot was recorded. Incidence
of callus and hyperhydricity occurrence in rooting cultures was also noted. Data were analyzed using a Mixed
Model of the statistical analysis software [19]. Means were separated using Tukey’s test and differences were
considered significant at p ≤ 0.05.