: In bone metabolism and the formation especially in bone substitution, calcium as basic module is of
high importance. Different studies have shown that the use of eggshells as a bone substitute material is a promising
and inexpensive alternative. In this in vitro study, the effects of eggshell granulate and calcium carbonate towards
primary bovine osteoblasts were investigated. Hyaluronan (HA) was used as artificial extracellular matrix (ECM) for the
used cells to facilitate proliferation and differentiation and to mimic the physiological requirements given by the egg
in vivo.