Current methods for the estimation of biogenic amines include thin layer chromatography, radioimmunoassay (RIA), enzyme-linked immunosorbent assay, high-performance liquid chromatography with ultraviolet detection (HPLC−UV), and high-performance liquid chromatography with mass spectrometry (HPLC−MS). However, HPLC with fluorescence detection is still the method of choice because of its high accuracy and sensitivity