Column Chromatography is based on a two phase system where the statooay phase is a column of adsorbant and the mobile phase is a liquid eluent. The common stationary phase usually is phase silica set or alumina. The stationary phase are placed into gass column. The sample is appled to the top of the column. The eluent then flows down through the column filled with the adsorbent. Under some conditions, the solute may be partitioning between an adsorbed solvent and the elution solvent, the partition coefficient, just as in the extraction process, determines efficiency of separation chromatography. The partition coefficient is determined by the solubility of the solute in the two phases We will isolate Lycopene from tomato paste using column Chromatography