assay Amylase was assayed to determine through the reducing sugarby the dinitrosalicylic (DNS) method, 0.2 ml of crude extract to1 ml of 1% soluble starch was added and then incubated for 30 minat 37◦C. On adding 1 ml of 3, 5 DNS, the reaction mixture wasboiled for 10 min. When it turned brown in color, the reaction wasstopped. The absorbance was measured at 540 nm. One unit (U) ofamylase was defined as the amount of enzyme that liberates 1 gof reducing sugars per minute under the assay conditions