To detect Staphylococcus aureus we studied a small black and shiny colony with oil zone, indicating
Staphylococcus aureus. A slide was prepared from an isolated colony and Gram staining method was applied. For
final verification of Staphylococcus aureus, citrated plasma was used to investigate the coagulase. S ,aureus catalase
test was positive, ability to ferment mannitol tests ,DNase was posetive, hemolytic zone on 5% sheep blood agar and
VP test were also performed.
For all the tests, S. aureus ATCC 25923 and S. aureus ATCC 29213 were used as negative control and positive control,
respectively (12).
To detect Staphylococcus aureus we studied a small black and shiny colony with oil zone, indicatingStaphylococcus aureus. A slide was prepared from an isolated colony and Gram staining method was applied. Forfinal verification of Staphylococcus aureus, citrated plasma was used to investigate the coagulase. S ,aureus catalasetest was positive, ability to ferment mannitol tests ,DNase was posetive, hemolytic zone on 5% sheep blood agar andVP test were also performed.For all the tests, S. aureus ATCC 25923 and S. aureus ATCC 29213 were used as negative control and positive control,respectively (12).
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