Following numerous studies on the influence of non-Saccharomyces yeast in winemaking, there has been a re-evaluation of the role of these yeasts. Indeed, some non-Saccharomyces yeast can enhance the analytical composition and aroma profile of the wine. In this context, over the last two decades, the use of controlled multi-starter fermentation using selected cultures of non-Saccharomyces and S. cerevisiae yeast strains has been encouraged 5, 6 and 7. Several aspects support the use of multi-starter fermentation in winemaking, the most important of which are: (i) modification of some specific analytical compounds, such as increased glycerol content, enhanced total acidity, or reduced acetic acid content of the wine; (ii) enhancement of the analytical profile of the wine (esters, volatile thyols); (iii) reduction of the ethanol content of the wine; (iv) control of the spoilage microflora in the wine; and (v) improvements to the overall quality and complexity of the wine