Individual FFAs were tested with re

Individual FFAs were tested with respect to their ANGPTL4 inducing capacity. As C4, C16, and C18:1 are the most abundant FAs in milk these FFAs were individually tested and C12 was included as an intermediate. To solubilise the LCFA, they were conjugated to BSA and for comparison C4 was treated the same way. BSAconjugated C12 and C18:1 increased ANGPTL4 mRNA in HCT 116 cells above the effect of BSA alone, whereas C4 and C16 had no additional effect compared with that of BSA (Fig. 7).