3.4 Zone of clearance on PLA plates To validate the screening process, the isolates MS-1, MS-2 and MS-3 were inoculated on to the emulsified PLA agar plates and we redetected for zone of clearance. After 20 days of incubation at mesophilic temperature i.e., 37°C, the isolates MS-1, MS-2 and MS-3 hydrolyzed the PLA and the diameter of the clearance zones were found to be