Reverse side pigments: The strains are divided into two groups according to their pigments on characteristic ability to produce the reverse side of the colony, called as and non distinctive or distinctive Colors observed for distinctive none were greenish brown, brownish black or distinct brown pigment. A color with low chroma such as pale yellow, olive or yellowish brown was included in the latter group In this study starch casein agar medium was used to grow the actinomycetes at 40°C for five days.