A postload wash of 4 CV binding buffer was performed before elution was conducted by either increasing the elution buffer in a single step to 100% or in a linear gradient to 100% over 30 CV.
A postloadwash of 4 CV binding buffer was performed before elutionwas conducted by either increasing the elution buffer in a singlestep to 100% or in a linear gradient to 100% over 30 CV.