Co2 papaya fruits were taken and it was completely peeled off. The pulp was macerated in mixie/blender and pasteurized at 85-90ºC for 5 minutes. A known volume of papaya juice was taken separately and potassium metabisulphite(KMS) at a rate of 200 ppm added to avoid growth of wild yeast and Diammonium orthophosphate at a rate of 0.2% was added as a source of nitrogen (N2) and phosphorus to yeast.