All extracts fromthe solid cultures were fractionated into
a highmolecularweight (protein) and a lowmolecularweight
(nonprotein) component on a Pharmacia PD-10 gel filtration
column using de-ionized water for elution, according to the
manufacturer’s recommendation. Each fraction was concentrated
by lyophilisation and examined for antifungal activity.
All the experiments performedwere in three independent
sets using triplicate plates.