Deposition of biomass Acetobacter xylinum centrifugation results are put into a solution of sodium alginate. A
number of 50 ml alginate solution prepared by mixing 1.5 gram alginate with 50 ml of distilled water. Then sterile
0.1 M CaCl2 prepared by mixing 1.47 grams of CaCl2 into the Erlenmeyer flask that had contained 100 ml of
distilled water for sterilization. For the next period of the cell precipitate in a solution of sodium alginate mixed in a
solution of sodium alginate as a trapper. Period of cell-alginate mixture that has been mixed evenly subsequently
dropped into 100 ml of sterile CaCl2 solution (in a glass beaker with a concentration of 0.1 M) were mixed on a
magnetic stirrer (Termolyne) on the speed with scale 1. To avoid the diversity of beads then chosen burette drops
with a capacity of 20 ml and ± 0.18 mm aperture size. Droplets of cell-alginate mixture in CaCl2 solution will soon
form a bead or beads immobilized cells, so automatically the time of Acetobacter xylinum cells already trapped in
Ca-alginate gel matrix. To form a solid beads immobilized cell, then left for ± 15 minutes in a solution of CaCl2.
Beads then immobilized cells obtained were then rinsed (2 times) with a 0.1% sterile peptone water.